Hello all. I purified some plasmids after doing precultures of strains from a 2-month old plate in -4 degrees. The PCR results were fine and I used theses plasmids to transform Agrobacterium cells. Should I be concerned about physiological changes for these strains from the old plate or even mutations concerning their genetic material ? (PS: I have the strains in glycerol too and I know that spread from glycerol stock should be a best practice).
I wouldn't use 2 month old plates unless there was no other option, personally.
I would vote to re-streak from the glycerol stocks too. Your older plates will have a lot of dead or unhealthy cells, may as well get some nice fresh materials going.
With such dishes, are you sure you have live bacterial cells? Not to mention the risks of contamination.
You must take a fresh culture, if you have no other option (culture stored in glycerol or in agar in a slanted tube), try to recover colonies and put them on a new dish, and if you have growth check the purity of your bacterial culture.
- Badges
- Science topic
- Similar topics
- Filing