Otávio Bravim

5 Questions 4 Answers 0 Followers

Questions related from Otávio Bravim

In Saccharonyces cerevisiae, the fatty acid purification removes the CoA?

I need to detect an oxoacyl in GC MS, but I wonder if the purification protocol of fatty acid described by Murad et al in the article Mass spectrometry characterisation of fatty acids from...

14 July 2016 656 0 View

Does anyone could suggest a Acetyl-CoA carboxylase assay in/for Saccharomyces?

I have transformant Saccharomyces, but I'm having trouble to find a good assay for detection of the ACC activity. Does anyone could suggest one?

30 June 2016 7,570 2 View

Does anyone know a lecithin molecule that can specifically binds to rhamnose?

I'm trying to express rhamnose and I need a way to detect its production through confocal microscopy and I saw in a meeting that some sugars can be detected by lecithin types. Does anyone could...

21 March 2016 6,222 1 View

Can anyone suggest a protocol for protoplast transformation for the transformation of Bacillus subtilis wild?

I have wild strains of Bacillus, and I'm with real problems in transforming them... I would like to try a protoplast transformation, can anybody suggest a optimized protocol??

03 August 2015 8,149 4 View

Does anyone have a Heat shock protocol for Bacillus subtilis H20.1, MT800 and 1012 and competent cells?

I'm having trouble to transform Bacillus subtilis in my lab, I tried some electroporation protocols, but they are not working. So I wonder if anyone would have a heat shock protocol I could use....

06 July 2015 7,764 2 View