I'd like to visualize zebrafish cilia, for which I'd need an antibody such as the one against γ-tubulin. Does anyone know any good ones?
Or if you might have other suggestions how to stain for zebrafish cilia, I'm open to suggestions!
Thank you very much
The most commonly used immunofluorescent probe for zebrafish cilia is the mouse monoclonal anti-acetylated alpha tubulin. Ciliary tubulin is acetylated on a lysine in most/all organisms, within a conserved sequence, whereas few cytoplasmic microtubules have acetylated alpha tubulin. The antibody, 6-11B-1, was first raised against sea urchin sperm axonemal tubulin by Gianni Piperno, and is widely available commercially.
For live imaging of zebrafish cilia, there is a GFP-tagged Arl13B that works well.
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