Hi,
My research will consist in examining the effect of compounds at concentrations of 10(-4)M, 10(-5)M, 10(-6)M, 10(-7)M, 10(-8)M on cells.
I prepared 100 mM stocks of compounds in 100% DMSO. Then I diluted the stocks so that after adding them to the wells of the plate, I obtained the following concentrations:
10(-4)M in 0.1% DMSO
10(-5)M in 0.01% DMSO
10(-6)M in 0.001% DMSO
10(-7)M in 0.0001% DMSO
10(-8)M in 0.00001% DMSO
Therefore, the final concentration of DMSO in the well of the plate did not exceed 0.1%. In this case, should I perform several vehicle controls for each DMSO concentration (0.1%, 0.01%, 0.001%, 0.0001%, 0.00001%)? However, is only one vehicle control enough, only for the highest concentration of DMSO in the well of the plate, i.e. 0.1%?
Or maybe I should prepare stocks of compounds so that there is the same concentration of DMSO in the wells of the plate, e.g. 0.1%?
I intend to publish the results in a journal. What should I do to make it good?
I will be very grateful for your help.
Regards
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