Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Can anyone explain why my starters are amplifying sequence from human genome instead of HPV16?

Hello, I'm working on primers from the publication: Okodo M, Okayama K, Teruya K, Sasagawa T. Uniplex E6/E7 PCR method detecting E6 or E7 genes in 39 human papillomavirus types. J Med Virol....

24 January 2021 7,636 3 View

Mounted sample stained with organic dyes (eg Alexa647) for superresolution?

I am looking for a way to prepare a mounted bio sample for testing superresolution methods, such as fixed cell culture. So far, most protocols I found for Alexa647 and other dyes require liquid...

12 January 2021 6,040 3 View

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04 January 2021 3,293 1 View

Can I add or delete studies from systematic review during revision stage of paper publication?

I have conducted a systematic review and one of the reviewers had mentioned about missing out on some good studies from the sample. on the surface the study is on a different topic but after an...

21 December 2020 573 4 View

What is the glucose content in Cellic CTec2?

Hi, I've determined the glucose content in Cellic CTec2 enzyme blend and it is 12-14 mg per 100 mg of enzyme blend. Could anyone share with me your results so I can compare it with mine?

08 December 2020 6,312 3 View

Paper authorship for students?

I had a few students help me with a simple but time-consuming task. The data they helped with will be used in a scientific paper. The students are part of the Student Research Program (SRP) at my...

11 October 2020 4,514 12 View

What is in common household dust?

Hi there, I am an undergraduate student in the UK and started to work on my dissertation, my subject is around common household dust. However, I can't seem to find any articles on basic and...

08 October 2020 6,838 1 View

I could not find a publication that fits the idea of evaporation together with DPD. Any suggestions?

I did find some papers that incorporate the two ideas together but I could not follow the research methodology. The simulation results were remarkable but not self explanatory. I tried to follow...

20 September 2020 5,354 4 View

How can I find out the molecular weight of acid chloride?

Dear all,, I am working on fatty acid chloride. So I wanted to find out the molecular weight of fatty acid chloride? So it is possible to find out using GPC or GCMS or there is any tritiometric...

06 September 2020 5,139 6 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View