How to culture primary patient-derived breast cancer cells?

06 July 2016 6,874 2 View

How to design gene specific gRNA for CRISPR/Cas9 system?

04 May 2016 3,784 3 View

Can anyone suggest an alternative method over western blot if no good antibody is available for a gene product i.e. the protein?

I have transfected tumor cells with pcDNA for a gene of interest. I have tried 5 antibodies for the same gene from different providers, but none of them worked nicely so far. I have mRNA...

02 March 2015 2,299 19 View

How can you find equally methylated regions in different samples using Infinium 450K array in R?

I have methylation data from Infinium 450K and I found out differentially methylated regions. Is there a way to find the equally methylated regions as well, particularly in R. Recommendation for...

10 November 2014 9,208 3 View

Which is the best and easiest R software package for infinium methylation 450K?

I am recently going to work on infinium methylation 450K array. I would like to analyze them using R for findign DMRs in my two groups. Can you suggest me which R software package would be easy...

01 February 2014 7,268 4 View

How can we check if our interested region comes under the known CpG Island or not?

I have next generation sequencing data and after validation came up with a few interested regions of the specific gene's promoter. However, I would like to know where and how can I check if my...

01 February 2014 6,287 4 View

How can we design customised siRNA targeted against our gene of interest?

I have little experience in siRNA designing.... 1) I looked at literature where some time only one sequence is mentioned and sometime sense and antisense sequence...Further sometimes ended with TT...

01 February 2014 7,660 11 View

How can you combine different published expression datasets and analyze them in R?

I would like to perform a in-silico validation for my research study where I need to combine some published datasets (from GEO portal) to increase the number of samples (n) and then analyse them...

01 February 2014 208 18 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View