Tushar Tomar

9 Questions 53 Answers 0 Followers

Questions related from Tushar Tomar

How to culture primary patient-derived breast cancer cells?

I am culturing cells from primary patient tumor in 2D using normal 10% FCS containing medium and 3D (mammosphere) using Mamocult medium. However they are in general slow growing. Any advise or...

07 July 2016 6,946 2 View

How to design gene specific gRNA for CRISPR/Cas9 system?

I have transfected cancer cell lines with pHAGE TRE dCas9-VP64 for overexpression. Now I need to select gene specific sequence for my guided-RNA. Any suggestion for specific and no off-target...

05 May 2016 3,891 3 View

Can anyone suggest an alternative method over western blot if no good antibody is available for a gene product i.e. the protein?

I have transfected tumor cells with pcDNA for a gene of interest. I have tried 5 antibodies for the same gene from different providers, but none of them worked nicely so far. I have mRNA...

03 March 2015 2,363 19 View

How can you find equally methylated regions in different samples using Infinium 450K array in R?

I have methylation data from Infinium 450K and I found out differentially methylated regions. Is there a way to find the equally methylated regions as well, particularly in R. Recommendation for...

11 November 2014 9,287 3 View

Can anyone share any experience for siRNA treatment for an in-vivo experiment on orthotropic cancer mouse model?

Would anyone like to share his/her experience on siRNA treatment for in-vivo experiment of cancer mouse model particularly for peritoneal cavity? I would like to know how frequent (daily or every...

03 March 2014 6,935 2 View

How can we design customised siRNA targeted against our gene of interest?

I have little experience in siRNA designing.... 1) I looked at literature where some time only one sequence is mentioned and sometime sense and antisense sequence...Further sometimes ended with TT...

02 February 2014 7,737 11 View

Which is the best and easiest R software package for infinium methylation 450K?

I am recently going to work on infinium methylation 450K array. I would like to analyze them using R for findign DMRs in my two groups. Can you suggest me which R software package would be easy...

02 February 2014 7,346 4 View

How can you combine different published expression datasets and analyze them in R?

I would like to perform a in-silico validation for my research study where I need to combine some published datasets (from GEO portal) to increase the number of samples (n) and then analyse them...

02 February 2014 330 18 View

How can we check if our interested region comes under the known CpG Island or not?

I have next generation sequencing data and after validation came up with a few interested regions of the specific gene's promoter. However, I would like to know where and how can I check if my...

02 February 2014 6,358 4 View