How to culture primary patient-derived breast cancer cells?

06 July 2016 6,874 2 View

How to design gene specific gRNA for CRISPR/Cas9 system?

04 May 2016 3,784 3 View

Can anyone suggest an alternative method over western blot if no good antibody is available for a gene product i.e. the protein?

I have transfected tumor cells with pcDNA for a gene of interest. I have tried 5 antibodies for the same gene from different providers, but none of them worked nicely so far. I have mRNA...

02 March 2015 2,299 19 View

How can you find equally methylated regions in different samples using Infinium 450K array in R?

I have methylation data from Infinium 450K and I found out differentially methylated regions. Is there a way to find the equally methylated regions as well, particularly in R. Recommendation for...

10 November 2014 9,208 3 View

Can anyone share any experience for siRNA treatment for an in-vivo experiment on orthotropic cancer mouse model?

Would anyone like to share his/her experience on siRNA treatment for in-vivo experiment of cancer mouse model particularly for peritoneal cavity? I would like to know how frequent (daily or every...

02 March 2014 6,864 2 View

How can we check if our interested region comes under the known CpG Island or not?

I have next generation sequencing data and after validation came up with a few interested regions of the specific gene's promoter. However, I would like to know where and how can I check if my...

01 February 2014 6,287 4 View

How can we design customised siRNA targeted against our gene of interest?

I have little experience in siRNA designing.... 1) I looked at literature where some time only one sequence is mentioned and sometime sense and antisense sequence...Further sometimes ended with TT...

01 February 2014 7,660 11 View

How can you combine different published expression datasets and analyze them in R?

I would like to perform a in-silico validation for my research study where I need to combine some published datasets (from GEO portal) to increase the number of samples (n) and then analyse them...

01 February 2014 208 18 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View