What is the right questionnaire in the investigation of Attention Grabbing Teacher Talks via Multimodal Metalanguage Methods?

Hi I wanted to work on a comparison between the traditional class discourse interaction analysis and the new discourse version interaction system caused by the virus, and I also wanted to work on...

20 November 2020 2,728 3 View

Do I need an ethics form for academic research if my case study does not include human data?

I am looking forward to do a case study on a government school in UAE. I will need to collect only technical data such as floor plans, electricity bills, interior pictures, lighting control data,...

29 August 2020 8,694 5 View

No title

The mixed_ethod type of writing in my Ph.D. project

17 February 2020 563 5 View

I am working with a protein and trying to resolve it on Blue-Native PAGE. Can somebody suggest be a recipe for it?

I have been trying to perform Blue-Native PAGE using procedure from doi:10.1038/nprot.2006.62. However, the stacking gel does not solidifies even after two hours, at room temperature. Can...

04 April 2019 4,777 2 View

I am planning for Native PAGE for a protein with pI 8.5 - 8.7. What should be the minimum difference of pH of buffer to avoid electrode switching?

The Gel has pH 8.9. and the pH of loading dye is 6.67. What should be the pH of running buffer to allow smooth running of my protein into the gel. I prepared buffer of pH 8.49 but I was unable to...

25 March 2019 9,877 6 View

SEM analysis of CaCO3 is good but EDX gives way different result than reported?

Hi I want to ask that i have a sample CaCO3 and when i analyze it using SEM i got result but using EDX Oxygen is about 50% which is very HIGH AS COMPARED TO LITERATURE AND CALCIUM % is low what...

11 October 2017 6,110 3 View

Sir I want to pelletized calcium oxide powder for my research I need a binder which would be stable at 1000C thanx ?

inert binder which would stable at 1000 C

28 April 2017 2,075 3 View

How can one extract the info data from .VCF file and merge it with the genotypes using R or any other tool?

08 November 2015 7,183 2 View

What would be the best solvent/buffer to dissolve a lyophilized peptide with a hydrophobicity gradient along the helix axis and the net charge of -1?

I have a 30 a.a. length lyophilized peptide which has a gradient of hydrophobicity along the helix axis and the net charge of -1 Which option is more important to determine the best solvent/buffer...

26 October 2015 8,888 6 View

How can we estimate soil moisture content from Landsat 8?

Soil moisture information is one indicator of drought and is the basis of action provision of irrigation water for plants to live optimally. Remote sensing technique is able to record accurately...

11 December 2014 1,191 20 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Irradiation data for JAPAN?

Hi, Could you help me to get the latest (2019, 2020) solar resource data for a particular site in JAPAN? PV GIS and NSRDB data are not in reach to JAPAN, I'm looking for some other database for...

03 March 2021 961 3 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View