Please I need procedure of plasmid extracted from bacterial cells using Promega's prepackaged kit?

More Nawar Alsaffawi's questions See All

Can PFAS be assessed on Agilient 6420 LCMS/MS without Jet Stream (AJS) ion source?

Quantitative Analysis for PFAS Compound

09 August 2023 538 2 View

How to extract DOI from scientific research?

03 March 2023 7,572 1 View

How to dissolve fly ash in a sample?what solvents can be used?

Hi I am trying to mix flyash in a sample having high CaO content what can be different solvents used to dissolve it?Can acetone, isopropanol, ethylene glycol and citric acid be used and how much?

01 March 2019 943 0 View

I want to know which investigation you do to diagnose unexplained recurrent implantation in your work ?

interested in your work and recurrent implantation failure.

21 January 2018 1,804 4 View

SEM analysis of CaCO3 is good but EDX gives way different result than reported?

Hi I want to ask that i have a sample CaCO3 and when i analyze it using SEM i got result but using EDX Oxygen is about 50% which is very HIGH AS COMPARED TO LITERATURE AND CALCIUM % is low what...

11 October 2017 6,274 3 View

Sir I want to pelletized calcium oxide powder for my research I need a binder which would be stable at 1000C thanx ?

inert binder which would stable at 1000 C

28 April 2017 2,220 3 View

Can anyone send me a recent research paper regarding different technologies used to capture sox and nox ?

the paper can have the efficiency of different technology, cost and other factors mentioned

18 April 2017 7,417 5 View

How can I calculate the weight of sorbent by using flow rate of gas and adsorption capacit of sorbent?

if the sorbtion capacity of any sorbent is give and flowrate of gas

23 February 2017 2,458 3 View

Is there any OVERALL score for the dbNSFP results?

I have a list of almost 1500 variants. I ran the search for the functional prediction using dbNSFP database. I got the results output file with the known 412 prediction columns. I wonder if there...

14 February 2016 6,690 0 View

How can one extract the info data from .VCF file and merge it with the genotypes using R or any other tool?

I have a huge VCF file with several GBs size. I can extract the genotypes for the required SNPs in a defined region using the VariantAnnotation package in R like this: rngs

08 November 2015 7,328 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

Where should the ARS sequence be introduced on a plasmid?

Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...

05 August 2024 1,573 4 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View