Do you have any idea/methods for in vitro flowering techniques? I am working on a highly regenerative invasive plant. I would like to try flowering in vitro.
We had a significative experience on Gentiana pneumonanthe. During the proliferation phase we obtained flowers and crops (as reported by the attached file). Our opinion is focused on effect of growth regulators added to the medium and the influence of genotype, combined to the light conditions, as reported in literature (Zhang e Leung, 2000)
Our experience with in vitro flowering of orchids we reduced N, increased Phosphate and potassium. please c our paper in Biol Plant 52(4): 725-726 (2008)
As we know, high nitrogen content in soil can induce and enhance growth rate of plant. After certain period or when the plant getting mature, introduction of high potassium content in soil can enhance the flowering rate of the plant. Same goes to in vitro studies, the nitrogen and potassium content in the media can influence the growth rate and also can induce flowering only for particular species.
Also you can try to use the paclobutrazol (PBZ) supplemented in the in vitro culture medium at the concentration of 1 mg/L or you must to test it to decide which one is the best concentration for your plant, good luck in your future work,
thanx sir but only few answers over 100 views ;), anyway (better than nothing :)
light quality seems little bit hanging, and how to do manipulation (to which direction?)... I ll try PBZ or medium formulationwith respect to N and K contents...
You can try to use GSH (1 mM) to induce flowering, but adjust pH before addition. Sugar os nalos very effective for flowering. induction. Good luck!
GSH mean Glutathione, 1 mM should be fine. There are many other possibilities to induce flowering, but this dependent from initial stage of the plants, internal hormones balance etc..
Is it possible to use seed to have shoot and do in vitro flowering on it?
We had a significative experience on Gentiana pneumonanthe. During the proliferation phase we obtained flowers and crops (as reported by the attached file). Our opinion is focused on effect of growth regulators added to the medium and the influence of genotype, combined to the light conditions, as reported in literature (Zhang e Leung, 2000)
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