I am looking for an equivalent of the MCF10A for breast for EMT-induction studies.
This is something that most of us working in the colon cancer field would need. There are several "normal colon epithelial cell lines" available from ATCC. How close to "normal" these are; I do not know. The best (albeit not easy) way would be to use primary cells or colonic crypt cultures.
CRL-1831 and NCM-460 are the normal colonic epithelial cell lines I could find.
ATCC has not mentioned on their page and the articles cited are not accessible . Are these cells CRL-1831 and CCD-18Co immortalised or normal primary cells?
Caco-2 cells are colon cancer cells and not normal. The CCD-18co are fibroblasts.
The human intestinal Caco-2 cell line has been extensively used over the last twenty years as a model of the intestinal barrier. The parental cell line, originally obtained from a human colon adenocarcinoma, undergoes in culture a process of spontaneous differentiation that leads to the formation of a monolayer of cells, expressing several morphological and functional characteristics of the mature enterocyte. Culture-related conditions were shown to influence the expression of these characteristics, in part due to the intrinsic heterogeneity of the parental cell line, leading to selection of sub-populations of cells becoming prominent in the culture. In addition, several clonal cell lines have been isolated from the parental line, exhibiting in general a more homogeneous expression of differentiation traits, while not always expressing all characteristics of the parental line. Culture-related conditions, as well as the different Caco-2 cell lines utilized in different laboratories, often make it extremely difficult to compare results in the literature. This review is aimed at summarizing recent, or previously unreviewed, data from the literature on the effects of culture-related factors and the influence of line sub-types (parental vs. different clonal lines) on the expression of differentiation traits important for the use of Caco-2 cells as a model of the absorptive and defensive properties of the intestinal mucosa. Since the use of Caco-2 cells has grown exponentially in recent years, it is particularly important to highlight these methodological aspects in order to promote the standardization and optimisation of this intestinal model.
FHC (ATCC® CRL-1831™)
https://www.lgcstandards-atcc.org/Products/All/CRL-1831.aspx?geo_country=gb#generalinformation
FHC (ATCC® CRL-1831™) are fetal cells and share many features with cancer cells.
NCM460 cells are immortalized cells from InCell. They are not "normal" cells.
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