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Questions related from Anchal Nigam
Hello, I am having difficulties to get smRNA FISH going on fresh frozen breast tissue. The technique worked well on MCF7 cells. We tried the same probes on tissue sample, and we haven't had...
06 June 2019 7,908 0 View
Does anyone have experience with bisuphite conversion of DNA from Breast FFPE sample. I have been using Qiagen direct Bisu FFPE kit with deparafinisation buffer . I am facing difficulties in...
03 March 2018 579 0 View
My plasmid carrying the insert is 5698bp and my host plasmid is 5737bp. I had done Xba1 and Sac1 digestion for both and had dephosphorylated my host. I had done the ligation in the molar ratio...
10 October 2014 3,532 16 View
I did SDM with double primers PCR. This was followed by dpn1 digestion.The gels showed amplicons but when I transformed there were no colonies. The polymerase used was pfu and Tm was 60 degrees...
01 January 2013 1,139 17 View
I would like to dock an enzyme with its substrate. Can anyone suggest any free software which I can use and which has a tutorial provided?
07 July 2012 6,814 22 View
I have been trying to do clustalx for my sequences of Polyketide synthase. Many of my sequences have two Acyl Carrier protein (ACP) domains. The problem is that clustal x is not accepting the...
06 June 2012 6,345 17 View
I am not getting my results for DNA isolation from Aspergillus. Can it be because the lysis buffer did not have SDS?
06 June 2012 1,148 7 View
