I'm trying to use OmpA as a control for my microscopy work as it's a major outer membrane protein, abundant in almost all bacterial strains. Is OmpA antibody commercially available? or does anyone used it before for any microscopy work?
E. coli OmpA antibody is available commercially. I have never used it myself:
http://www.antibodyresearch.com/ompa-arc111120.html
I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...
08 September 2018 6,520 0 View
I have a T-cell line that expresses Human TCR and I've being using anti-human TCR α/β (Clone IP26) to detect the TCR expressing cell population using Flow Cytometry. However, this antibody hasn't...
08 September 2018 7,813 3 View
Can anyone give me a simple explanation for the function of T-cell-receptor-like antibodies? How do they mimic TCRs?
05 June 2018 3,151 2 View
I'm using amber suppressor tRNA in E.coli to perform in vivo photo-cross-linking experiments to study certain protein-protein interactions at the outer membrane. I do get indications of...
09 October 2016 2,051 6 View
I'm trying map-out interaction sites of two protein units sitting on the same protein super-complex. They are well known to interact with each other, but the PDB structures I'm looking at have...
05 June 2016 3,960 4 View
I am trying to understand the difference between a Knockout strain and a Deletion strain.
08 September 2014 1,181 11 View
I am performing a protein localization experiment on E.coli using a complimented strain which has a pBAD vector. I need to get this plasmid based expression to native protein level. Also I am...
07 August 2014 3,469 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?
06 August 2024 9,618 3 View