Has anyone worked with 5KC T-cell Hybridoma (Mouse) cell line before?

I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...

08 September 2018 6,378 0 View

No title

08 September 2018 7,716 3 View

What are T-cell-receptor-like antibodies?

Can anyone give me a simple explanation for the function of T-cell-receptor-like antibodies? How do they mimic TCRs?

05 June 2018 3,065 2 View

How to enhance your in vivo photo-cross-linking experiments in E.coli?

09 October 2016 1,952 6 View

How do you fuse two PDB structures which contain two different proteins of the same protein super-complex and load it in Pymol?

I'm trying map-out interaction sites of two protein units sitting on the same protein super-complex. They are well known to interact with each other, but the PDB structures I'm looking at have...

05 June 2016 3,846 4 View

Are the Knockout strains and Deletion strains the same in the Genetic terminology?

I am trying to understand the difference between a Knockout strain and a Deletion strain.

08 September 2014 1,084 11 View

Can anyone please suggest a method to tune up plasmid expression level to native levels?

I am performing a protein localization experiment on E.coli using a complimented strain which has a pBAD vector. I need to get this plasmid based expression to native protein level. Also I am...

07 August 2014 3,389 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View