Has anyone worked with 5KC T-cell Hybridoma (Mouse) cell line before?

I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...

08 September 2018 6,378 0 View

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08 September 2018 7,716 3 View

How to enhance your in vivo photo-cross-linking experiments in E.coli?

09 October 2016 1,952 6 View

How do you fuse two PDB structures which contain two different proteins of the same protein super-complex and load it in Pymol?

I'm trying map-out interaction sites of two protein units sitting on the same protein super-complex. They are well known to interact with each other, but the PDB structures I'm looking at have...

05 June 2016 3,846 4 View

Can anyone recommend a good OmpA antibody that can be used for Immunofluorescence experiments, specifically for E.coli staining?

I'm trying to use OmpA as a control for my microscopy work as it's a major outer membrane protein, abundant in almost all bacterial strains. Is OmpA antibody commercially available? or does anyone...

02 March 2015 4,451 1 View

Are the Knockout strains and Deletion strains the same in the Genetic terminology?

I am trying to understand the difference between a Knockout strain and a Deletion strain.

08 September 2014 1,084 11 View

Can anyone please suggest a method to tune up plasmid expression level to native levels?

I am performing a protein localization experiment on E.coli using a complimented strain which has a pBAD vector. I need to get this plasmid based expression to native protein level. Also I am...

07 August 2014 3,389 3 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

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03 March 2021 5,360 2 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View