Dilshan Gunasinghe

8 Questions 16 Answers 0 Followers

Questions related from Dilshan Gunasinghe

Has anyone worked with 5KC T-cell Hybridoma (Mouse) cell line before?

I am using 5KC T-cell Hybridoma cell line in one of my T-cell receptor (TCR) studies and was wondering whether anyone have more information about their immunological characteristics. Like what...

09 September 2018 6,469 0 View

Any recommendations for a suitable anti-Human TCR αβ antibody works for Flow Cytometry and Microscopy?

I have a T-cell line that expresses Human TCR and I've being using anti-human TCR α/β (Clone IP26) to detect the TCR expressing cell population using Flow Cytometry. However, this antibody hasn't...

09 September 2018 7,769 3 View

What are T-cell-receptor-like antibodies?

Can anyone give me a simple explanation for the function of T-cell-receptor-like antibodies? How do they mimic TCRs?

06 June 2018 3,118 2 View

How to enhance your in vivo photo-cross-linking experiments in E.coli?

I'm using amber suppressor tRNA in E.coli to perform in vivo photo-cross-linking experiments to study certain protein-protein interactions at the outer membrane. I do get indications of...

10 October 2016 2,012 6 View

How do you fuse two PDB structures which contain two different proteins of the same protein super-complex and load it in Pymol?

I'm trying map-out interaction sites of two protein units sitting on the same protein super-complex. They are well known to interact with each other, but the PDB structures I'm looking at have...

06 June 2016 3,920 4 View

Can anyone recommend a good OmpA antibody that can be used for Immunofluorescence experiments, specifically for E.coli staining?

I'm trying to use OmpA as a control for my microscopy work as it's a major outer membrane protein, abundant in almost all bacterial strains. Is OmpA antibody commercially available? or does anyone...

03 March 2015 4,535 1 View

Are the Knockout strains and Deletion strains the same in the Genetic terminology?

I am trying to understand the difference between a Knockout strain and a Deletion strain.

09 September 2014 1,138 11 View

Can anyone please suggest a method to tune up plasmid expression level to native levels?

I am performing a protein localization experiment on E.coli using a complimented strain which has a pBAD vector. I need to get this plasmid based expression to native protein level. Also I am...

08 August 2014 3,440 3 View