Hi there. There are a few protocols around for sure and on the face of it they appear quite straight forward (i.e. fixing, hypotonic solution, then dropping onto slides), however the analysis is far from easy. It takes 3 to 4 years training to be an experienced and registered clinical cytogeneticist. I tried myself but soon realised I needed expert advice (bits of chromosomes here and there etc). I suggest you outsource to a clinical genetics laboratory  rather than doing this by yourself.

Best wishes,

Thank you sir Maurice Canham for your reply. I have experience in clinical cytogenetics  but now im working in a research facility and i need to karyotype stem cells specially mesenchymal. i performed a protocol for human embryonic stem cells on the mesenchymal and the results were encouraging. im wondering if you know a special protocol for mesenchymal stem cells please provide me with it.

thanks again

Hi there, apologies for the late reply. I don't know of a protocol specific for mesenchymal stem cells however I'd guess that a protocol suitable for ES cells or indeed mammalian cells generally may be suitable.

Hi, Making of Chromosomal spreads it's easy right now: http://www.ebay.com/itm/-/182431736298?

Hi Andrey Cheremin,

did you try this instrument, if yes does it give you  a good spreaded meta-phase without cytoplasm or over lapping