Can this pH of 8 be due to the presence of other substances? Or do I have an error somewhere in the analysis? Thank you!
26 June 2024 9,093 4 View
Dear colleagues, I am looking for advice on the validation of a standard questionnaire that I intend to translate. The original version contains 40 items. Could you please tell me what sample...
26 March 2024 1,660 11 View
So I have ben trying to prepare TiO2 nanoparticles but i am getting trouble with the fromation of White PPT which later dissolves to form a clear solution that unfortunately doesnt form a gel. it...
25 March 2024 8,808 2 View
For both heat-induced or proteolytic-induced antigen retrieval, does the AR needs to be done on the same day of the IF? Or can be done one day in advance, store the slides at 4º, and performe the...
06 March 2024 220 1 View
Any empirical study about theater education for immigrant students?
03 March 2024 6,134 0 View
Currently, I am trying to model packers made from recycled plastic material (E=360N/mm^2 and a poisson ratio of 0.4). The best way to model these packers would be to only adjust the contact...
26 February 2024 5,699 1 View
Optimal buffer and conditions to determine the enzymatic activity of MPO by the colorimetric method that is based on the formation of hypochlrous acid which reacts with taurine to form the...
07 February 2024 3,055 0 View
In Guassian, which convergence algorithm is best for working with transition metal interaction? It is taking a long time to even get the first block of coordinates, in the output file it said to...
28 January 2024 4,276 1 View
I need to evaluate abdominal adipose tissue hypertrophy in a murine model with metabolic syndrome, with and without treatment and I need to review which is the best objective to measure adipocyte...
09 January 2024 7,149 0 View
I am combining drug A (IC20 and IC50) with drug B (5 uM, 10 uM and 50 uM). Drug B reduces cell viability to 28% at 50 uM, while drug A IC20 combined with drug B 50 uM reduces cell viability to...
01 January 2024 5,749 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,081 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,822 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,155 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,104 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 639 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,706 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View