02 June 2024 0 2K Report

What is the specific cucumber (Cucumis sativus) biomarker for standerdization and fingerprinting using HPLC.

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If i am creat a hybrid composite where the reinforcement Egg shell and SiC then what will be the wear trend?

Hybrid composite matrix -Aluminium Alloy Reinforcement - Egg shell and SiC

29 March 2024 5,649 6 View

Role of zinc as an antioxidant?

As an antioxidant role of zinc, conflicting reports are pouring. It is recognized that Zn has prominent role in reducing oxidative stress by scavenging and decreasing production of ROS. On the...

19 June 2023 9,769 1 View

Can anyone let me know how to extract tRNA from adherent cells?

I want to isolate tRNA from adherent cells (DLD1) which will be used for further screening assays. is there any kit available or any protocol that I need to follow? Its a bit urgent. Thanks in...

03 March 2023 3,084 1 View

If anyone can suggest for any cDNA synthesis kit for small RNAs such as miRNA as well as piRNA, which has a shippment to india ?

Usually there are cDNA synthesis kit for microRNA but not for piRNA , previously we were using miScript RT II kit , but the product has been discontinued for now .If anyone can suggest any ideas.

08 January 2023 1,743 6 View

What is the acceptable range of bacterial endotoxin in purified recombinant in EU/ml?

Can anyone help me know what I should expect as an acceptable range of endotoxin in EU/ml from my purified recombinant protein sample, purified using E.coli expression vector.

21 November 2022 1,593 4 View

How to express recombinant gamma-synuclein using BL21 cells?

I am trying to express gamma-synuclein using E. coli BL21 cells. Antibiotic conc: 50mg/ml, IPTG 1mM for 4 hrs, 37 degrees. After sonication, boiling water bath is used before cell lysate...

27 June 2022 8,638 1 View

Can you have a single goodness of fit measure for multiple regression analysis or a number of measures to be taken to explain performance?

Should we use a single goodness of fit measure like R square for evaluating performance of multiple regression application or a number of measures like R-square, adj R square, AIC, BIC, RMSE etc....

20 October 2021 3,249 5 View

Can any body give any open source link for getting important stock prices of important global hospitality firms and restaurants?

Can any body give any open source links for downloading stock prices of important global hospitality firms and restaurants? The data should be daily/weekly stock price from August 2019 till...

01 October 2021 9,689 2 View

Why Shri Lal Pal has been included in our published paper?

Development of bioconcrete material using an enrichment culture of novel thermophilic anaerobic bacteria May 2006 Indian Journal of Experimental Biology 44(4):336-9 P Ghosh, Saroj Mandal. S Pal, G...

01 October 2021 231 1 View

What are the opensource Partial Least Squares (PLS) tools available?

Can anybody share opensource Partial Least Squares (PLS) tools that is easy to handle, reliable and easy to learn analysis and interpretation?

16 September 2021 9,692 6 View

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Which type of compound does lamda max of 218 indicate in a uv-vis spectrum of a partially purified compound through column and TLC?

A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...

11 August 2024 9,801 2 View

Can you connect an HPLC to a Mass Spec only at a certain time point?

Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...

11 August 2024 8,141 3 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

Separation of organic acids-HPLC?

Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.

07 August 2024 8,782 3 View

Why is electronic energy 0.000 for liquid crystal compounds and is invariable with temperature in Guassian 09 software?

Are there any suggestions or insights you can provide to help address this problem

06 August 2024 1,443 2 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Why is TLC not a form of partition chromatography?

Why is TLC not a form of partition chromatography and paper chromatography not a form of  absorption chromatography?

03 August 2024 7,035 3 View

Is there any Indian Company or Startups, from any universiy or IITS, which manufacture Gas Chromatography instrument , i want to make a custOmized G?

or we can collaborate

01 August 2024 6,705 2 View

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