Can anyone help with mammospheres aggregation?

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Goodwin G. Jinesh

Aggregation is characteristic to sphere forming cells. Once you inhibit aggregation, there wont be mammosphere formation. See my paper below, which might give you some clues about which agents to use. I guess heparin is one good candidate.

https://www.researchgate.net/publication/233742776_Blebbishields_the_Emergency_Program_for_Cancer_Stem_Cells_Sphere_Formation_and_Tumorigenesis_after_Apoptosis

Article Blebbishields, the Emergency Program for Cancer Stem Cells: ...

Olivia B Harris

Matrigel and BME are used in other organoid systems - you could try giving these a go. Hope this helps.