I need to culture an E0771 cell line. I know I should use RPMI 1640 with serum and 10 mM Hepes. Does it need any other supplementation?
Serum level between 5% and 10%...
You can add the well-known tags like "cell culture" , "cancer research" result in more researcher will observe your question to be get better answers.
I'm looking for specific markers to identify Mel270 and OMM1.3 cell lines, can anyone help?
04 May 2015 8,123 1 View
I need to obtain cell lysates in order to perform Western Blots to check the presence od MMP-2 and MMP-9. What would be the best lysis buffer?
09 October 2014 7,155 5 View
What's the best media for Mel-270 cell line? How fast do the cells grow?
08 September 2014 6,326 3 View
08 September 2014 5,129 3 View
24 November 2013 7,154 2 View
I would like to inject E0771 cell line in, eg. back leg of mouse. Will it grow nicely or not? Has someone ever tried that?
04 May 2013 3,886 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.
08 August 2024 3,746 4 View
May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/
08 August 2024 4,585 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...
07 August 2024 4,600 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...
04 August 2024 4,130 4 View