Does anyone know what are the surface markers for melanoma cells Mel270 and OMM1.3?

I'm looking for specific markers to identify Mel270 and OMM1.3 cell lines, can anyone help?

04 May 2015 8,085 1 View

What's the best lysis buffer to obtain MMP-2 and MMP-9 from cell lysates?

I need to obtain cell lysates in order to perform Western Blots to check the presence od MMP-2 and MMP-9. What would be the best lysis buffer?

09 October 2014 7,115 5 View

Mel-270 cell line

What's the best media for Mel-270 cell line? How fast do the cells grow?

08 September 2014 6,279 3 View

Mel-270 cell line

What's the best media for Mel-270 cell line? How fast do the cells grow?

08 September 2014 5,079 3 View

Can anyone help with E0771 maintenance?

I need to culture an E0771 cell line. I know I should use RPMI 1640 with serum and 10 mM Hepes. Does it need any other supplementation?

24 November 2013 7,099 2 View

Can the tumor from E0771 cell line grow in other sites than the orthotropic site?

I would like to inject E0771 cell line in, eg. back leg of mouse. Will it grow nicely or not? Has someone ever tried that?

04 May 2013 3,832 3 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View