Ham’s F-12 medium containing 10% heat-inactivated FBS , 2 mM L-glutamine, 10 mM HEPES buffer solution, 1% nonessential amino acids solution, 1% penicillin-streptomycin-Fungizone solution (BioWhittaker), and 1 mM sodium pyruvate . Mel 270, can be maintained in complete RPMI 1640 containing the same additives as the Ham’s F-12 with the addition of 5 × 10−5 M 2-ME.