Can the tumor from E0771 cell line grow in other sites than the orthotropic site?

More Katarzyna Jasińska-Konior's questions See All

Does anyone know what are the surface markers for melanoma cells Mel270 and OMM1.3?

I'm looking for specific markers to identify Mel270 and OMM1.3 cell lines, can anyone help?

04 May 2015 8,123 1 View

What's the best lysis buffer to obtain MMP-2 and MMP-9 from cell lysates?

I need to obtain cell lysates in order to perform Western Blots to check the presence od MMP-2 and MMP-9. What would be the best lysis buffer?

09 October 2014 7,155 5 View

Mel-270 cell line

What's the best media for Mel-270 cell line? How fast do the cells grow?

08 September 2014 6,326 3 View

Mel-270 cell line

What's the best media for Mel-270 cell line? How fast do the cells grow?

08 September 2014 5,129 3 View

Can anyone help with E0771 maintenance?

I need to culture an E0771 cell line. I know I should use RPMI 1640 with serum and 10 mM Hepes. Does it need any other supplementation?

24 November 2013 7,154 2 View

Can anyone help with E0771 maintenance?

I need to culture an E0771 cell line. I know I should use RPMI 1640 with serum and 10 mM Hepes. Does it need any other supplementation?

10 November 2013 3,211 2 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

May members post flyers about opportunities to present at a conference? If so, where to post?

May members post flyers about opportunities to present at a conferehttps://veraeducation.com/nce? If so, where to post for the Virginia Educational Research Association? https://veraeducation.com/

08 August 2024 4,585 1 View

Hello all, Looking for international reviewer to review Ph.D thesis in wireless sensor network.Can anybody help?

My name is Apurva Saoji. I am a Ph.D scholar in Computer engineering in India. I am looking for international expert in reviewing my PhD thesis, "Competitive Optimization Techniques to Minimize...

07 August 2024 4,600 2 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to understand this crystallographic phenomenon of low temperature crystals in zeolite?

During low-temperature testing, new diffraction peaks that appear could be indicative of several phenomena. In one of our tests, we observed notable new peaks around 40° and 45° in a specific...

06 August 2024 726 3 View

Research Methodology - Impact of Corporate Reputation on Stakeholders Behaviors?

Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks

06 August 2024 949 0 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

04 August 2024 4,130 4 View

Ulrich Pfeffer

No I don't but you can grow most cell lines in any place if you inject it together with Matrigel. http://www.ncbi.nlm.nih.gov/pubmed/1521911

Joanne Y H Lim

I have injected E0771 in the thigh muscle of C57BL/6 mice and it grows well. I injected 100,000 cells and tumors were palpable by day 13.

Shahzad Nawaz Syed

Hi Joanne,

Did you see metastasis with these cell in mouse? I mean can you isolated back these cells from other organs such as lung, liver or spleen of injected mouse?