Does anyone know what are the surface markers for melanoma cells Mel270 and OMM1.3?

I'm looking for specific markers to identify Mel270 and OMM1.3 cell lines, can anyone help?

04 May 2015 8,085 1 View

What's the best lysis buffer to obtain MMP-2 and MMP-9 from cell lysates?

I need to obtain cell lysates in order to perform Western Blots to check the presence od MMP-2 and MMP-9. What would be the best lysis buffer?

09 October 2014 7,115 5 View

Mel-270 cell line

What's the best media for Mel-270 cell line? How fast do the cells grow?

08 September 2014 5,079 3 View

Can anyone help with E0771 maintenance?

I need to culture an E0771 cell line. I know I should use RPMI 1640 with serum and 10 mM Hepes. Does it need any other supplementation?

24 November 2013 7,099 2 View

Can anyone help with E0771 maintenance?

I need to culture an E0771 cell line. I know I should use RPMI 1640 with serum and 10 mM Hepes. Does it need any other supplementation?

10 November 2013 3,166 2 View

Can the tumor from E0771 cell line grow in other sites than the orthotropic site?

I would like to inject E0771 cell line in, eg. back leg of mouse. Will it grow nicely or not? Has someone ever tried that?

04 May 2013 3,832 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View