What is the best model to be use for site prioritization using GIS-MCA interface?

What model can be use to identify prority risk site using multicriteria analysis and GIS techniques

05 January 2021 9,626 3 View

What are suggested time frames sufficient for different stages of a biotechnology project?

The project will include; PBMC isolation from whole blood ThP1 maturation to macrophages Challenge PBMC and ThP1 derived macrophages with LPS + test peptide qPCR of IL6, IL8 and TNF ELISA for IL6,...

23 April 2017 5,597 1 View

What are efficient proliferation assays for peripheral blood mono nuclear cells, and cytokine release. This is to test the effect of a peptide on PBMC

The project involves determining the effect of a certain peptide on PBMC, i require information on efficient assays that can answer this question.

18 March 2017 6,305 5 View

Can anyone tell me the best range of concentrations in working with antimicrobial agents?

I am working with some fern antimicrobial properties and I would like to know the range of the best concentrations that I can prepare my plant extracts before I can be sure they have performed...

15 April 2014 153 10 View

Physics difficulties to students

Why is it that majority of high school students do not like Physics? Majority of Undergraduates science-related students also dislike Physics, why? Is it that Physics teachers/tutors/lecturers are...

01 January 1970 4,797 3 View

Are there any recent research questions in smart concrete technology?

I am seeking to find possible research questions on smart concrete for PhD

01 January 1970 8,274 3 View

Please how do i make biosynthesized silver nanoparticles devoid of impurities as much as possible?

I want to know the best method to use in washing biosynthesized silver nanoparticles to achieve the best purity level before it is taken for instrumental characterization.

01 January 1970 4,835 3 View

Undergraduates Interest in Physics related Courses

Dear colleagues, may you please suggest methods to improve the interest of undergraduate students in Physics related and general science courses.

01 January 1970 1,312 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View