What is the best model to be use for site prioritization using GIS-MCA interface?

What model can be use to identify prority risk site using multicriteria analysis and GIS techniques

05 January 2021 9,626 3 View

What is the best software for experimental design using Taguchi method?

Taguchi method have been used widely by researchers in the recent times due to its less number of experimental runs advantage compared to full factorial design and response surface method. For...

31 March 2020 2,260 14 View

How can we optimize the morlet wavelet parametrs using genetic algorithm?

Dear researchers Looking forward to your support to the above issue. I want to do implementation in matlab. Anyone with source codes and reference materials, please help me. Thanks

05 January 2020 5,836 4 View

Lookind for a Public Health research topic in Zim for a Phd programme any assist please inbox?

All public health topics welcome

10 November 2019 8,556 3 View

How do we convert machine vibration signals to vibration images?

Hello dear researchers. I am doing a research in fault diagnosis of rolling element bearings. I am stacked on how to convert the vibration signals to vibration images. I would appreciate for any...

19 September 2019 9,197 2 View

I need a methodology for investigating the source rock of a placer gold deposit?

Which methodology should i use to trace/investigate the source rock or geological structure responsible for placer gold deposit in an artisanal mining site?

25 January 2019 3,677 13 View

Immunofluorescence staining with streptavidin micropaticpes bind to flurophore biotin?

Hi, Any one please help me to get a protocol for immunofluorescence staining of streptavidin coated particles bind to biotin fluorescent coated. I couldn't find an article related to that....

02 January 2019 4,530 1 View

Can anyone help with a protocol for making cigarette smoke extract in cell culture medium?

I am in serious need of a protocol for making cigarette smoke extract. I will be exposing epithelial cell line to this cigarette smoke extract. It would be very helpful if this protocol can be...

11 November 2018 5,006 3 View

How to do statistical analysis?

Hi All, we would like to know the statistical analysis of an experiment . We have done two different methods in 5 samples(different sizes as x axis) and got yield of these two methods for each...

05 December 2017 9,377 6 View

Concentration of nano particle solution ?

22 August 2017 6,013 4 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

What software programs can directly simulate Additive Manufacturing Directed Energy Deposition (DED)?

I understand there are a lot of software programs that can simulate DED by going through specific modeling, simulation, and commands. For me, that was time consuming and results were not accurate....

24 February 2021 4,428 3 View