Hi, I have some left over spin columns from my gel extraction kit. I was wondering if I could use this with my RNA extraction buffer to extract RNA? Has anyone tried this?
Yes, you can use it. I have the same experience and it had been worked for me as well. Because the column is for Nucleotide acid traping.
Hello Abiola Afonja,
I have tried this in the past while isolating plasmid DNA as well as bacterial RNA. In fact, I used columns from different companies and it worked well for me.
The spin column is made up of a silica membrane that is designed to capture the nucleic acid (be it RNA or DNA). It would work the same way in your case as well.
The efficiency might differ but the column can be used without any problems.
Hello Abiola Afonja
Spin columns are designed to work optimally within its own kit format, but you can still use these columns for RNA extraction. You can try a pilot run and check for yourself. I agree with Abhijeet Singh statement that the efficiency might differ.
I have attached a research article for your reference for more information on this topic.
https://www.nature.com/articles/s41598-018-30316-w
Best.
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