9 Questions 7 Answers 0 Followers
Questions related from Abiola Afonja
Hi, I have some left over spin columns from my gel extraction kit. I was wondering if I could use this with my RNA extraction buffer to extract RNA? Has anyone tried this?
14 December 2021 5,808 4 View
Hi, I'm running some immunofluorescence next week and wanted to sterilize my cover slips in 24 well plates in advance. Does anyone know if I should leave the plates open under uv light or I can...
10 December 2021 8,857 4 View
I'm trying to clone some cDNA, but when I PCR the cDNA with my primers, I get no product on the gel. So I checked around and found out that I might be missing the second strand synthesis step,...
09 December 2021 4,327 3 View
I need some help with a gene cloning protocol. Which is used as template for insert into the plasmid, gDNA or cDNA?
20 October 2021 5,965 5 View
I am doing some qPCR and noticed that ct values for genes TNF, DPP10 and KCND2 are in the range 30s, I've tried a different cDNA kit which allows me use a total RNA of 5mcg and that has helped get...
30 March 2021 8,123 4 View
I am in serious need of a protocol for making cigarette smoke extract. I will be exposing epithelial cell line to this cigarette smoke extract. It would be very helpful if this protocol can be...
12 November 2018 5,114 3 View
Hello, I'm doing a smoking study on BEAS-2B cells, and was wondering what the most appropriate method of preparing cigarette smoke extract in the lab, I do not have access to a smoke machine....
23 October 2018 3,008 1 View
The project will include; PBMC isolation from whole blood ThP1 maturation to macrophages Challenge PBMC and ThP1 derived macrophages with LPS + test peptide qPCR of IL6, IL8 and TNF ELISA for IL6,...
24 April 2017 5,699 1 View
The project involves determining the effect of a certain peptide on PBMC, i require information on efficient assays that can answer this question.
19 March 2017 6,410 5 View
