Antibody circles forming in cell immunofluorescence?

02 June 2024 1 7K Report

Hi, I sometimes get these circles forming in my cell IF. They do not always show up, but sometimes I can't seem to avoid them even when I follow the same protocol. Any ideas what may be causing this issue?

The red signal is supposed to be cox4. I have included a reference photo to show the expected staining pattern.

Ellen A G Chernoff

Perhaps there is an intermittent source of oxidative stress. See figure 2.

https://elifesciences.org/articles/76095

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