Why i am facing problem during sds page given in the below photos?

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Does the pressure(-600 mmHg) affects pH?

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After a reference from Int J Body Compos Res, can any body help?

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Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

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I am interested in medicinal chemistry field, which books are highly recommended to learn from?

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Do anyone here can help us in the field of molecular docking?

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Does anyone have kinetic and kinematic dataset for gait classification?

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SDS page software for analysis?

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Do I need to strip the PVDF membrane before staining it with colloidal gold?

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Bakunovich Juliya

Hi. Maybe the voltage is affected by the solution itself? Or how clean is the container in which you are conducting the experiment?

Jubayer Ahmed

My voltage set for free..I set constant current but current fluctuates

Deepanshi Nara

Hi, I have had these humps in my gels. These could be a result of different environment inside and outside the cassette. For me- readjusting the pH of the running buffer and using a freshly made buffer resolved the issue. If reusing the buffer then checking the pH and mixing it uniformly before using for next cycle also help.