How to best represent qPCR DD Ct or D Ct as a box plot?

I've been reading up on how to best represent qPCR data, specially on answers given on this site by Dr. Jochen Wilhelm. I understand box plots are the way to go, but I still don't understand them...

11 February 2021 7,228 3 View

How can I improve a DNA extraction from serum?

Hi everyone! I'm working on a DNA extraction from serum. Unfortunately the concentration was very low and the PCR amplification didn't work, maybe because the DNA extracted was very fragmented. Do...

23 August 2018 4,155 3 View

FAME CG analysis (derivation) ?

29 October 2017 9,586 3 View

Could someone please help me identify autofluorescent structures in salamander embryos?

Hello, I have fixed and obtained frozen sections of salamander embryos for fluorescence microscopy. The sections are 8um thick and the embryo used for the attached images was kidney bean shaped...

13 February 2017 7,604 1 View

Protocol for Immuno TEM?

Hello everyone,I am gearing up to prepare my first ImmunoTEM samples via a pre-embedding protocol. The samples are isolated mouse sperm. I searched the literature and I am gathering bits and...

03 March 2016 9,794 5 View

Where can I buy the standards of quercetin and gallic acid for a thesis?

02 July 2014 4,690 3 View

Does anyone know about the Sevag method for protein removal?

I am extracting Lentinan from Shiitake and the method says that I need to remove protein usin the Sevag method. I have searched and the only thing that I have found is the usage of chloroform and...

03 December 2012 5,187 5 View

The TLR4 is an option in obesity research?

The TLR4 is part of system inmune and recognizes LPS of the microorganism. Saludos

28 December 2011 4,818 3 View

How many people/families currently work on informal e-Waste recycling in the world?

A lot of papers and reports suggests that thousands or millions of people or families work as informal "recyclers" in the world, or at least in developing countries. Nevertheless, it is not clear...

01 January 1970 4,906 3 View

*WESTERN BLOT* How do you transfer high and low MW proteins at the same time? I'm trying to get Claudin-5 (~22kDa) and ZO-1 (~220kDa)

I run the electrophoresis in a pre-cast 4-20% gel at 200 V for ~45 min, then i've tried to transfer them to nitrocellulose membrane in the following conditions (always in a cold, wet environment):...

01 January 1970 7,229 6 View

What is the procedure to determine the permeability of concrete?

can anyone help me in this, it will help me with my research work

01 March 2021 834 3 View

Mixed ANOVA with some normal and some non-normal data?

I am looking to run a 2x5 Mixed ANOVA to examine Sensory Processing Sensitivity in Highly Sensitive People (HSP) and Non_HSP. My Between-Subjects factor is HSP and Non-HSP. All participants tested...

11 February 2021 1,445 6 View

How to model in HFSS or FEKO Free Space Material Characterization?

I am having trouble setting up this problem in HFSS or FEKO. In HFSS, I've tried both FEM and FEBI-IE solvers. With FEKO, I've tried it's MoM solver and default settings. I am would like model...

09 February 2021 375 2 View

How can I convert permeability unit from barrer to m^2 for using COMSOL MultiPhysics?

I am looking for gas permeability of PDMS for simulation in COMSOL MultiPhysics and using creeping flow , fluid and matrix properties. The permeability unit in COMSOL is m^2 whereas I have the...

08 February 2021 4,305 2 View

Can I simulate Ferrite in HFSS?

I have a design with 2 dipoles working at different frequencies joined in one system and separated by Ferrite. I think, Ferrite was used to minimize the interference between 2 antennas as much as...

25 January 2021 5,918 3 View

How to quantify how much of a fluorescent dye is entering a cell?

I'm looking to compare a few fluorescent dyes for cell permeability. I'm interested to see how much of the dye actually enters the cell, but would specifically like to quantify this data. Any...

21 January 2021 6,089 3 View

Permeability of fractured reservoirs?

Hi all, What I know the permeability of matrix and fracture can be in a broad range in fractured reservoirs. Am looking for the most usual range for their permeability separately. I will be highly...

15 January 2021 5,271 2 View

How to make an apparatus for testing membrane permeability and selectivity?

Good afternoon everyone! I just can't find drawings or a description of the creation of an apparatus for determining the permeability and selectivity of membranes in relation to gases and gas...

14 January 2021 9,205 4 View

Can I use saponin to permeabilize cell to measure mitochondrial respiration?

Saponin and digitonin both can permeabilize cells. However, based on many respiration assay protocols I read, saponin is used for tissue permeabilization and digitonin is used for cell...

11 January 2021 2,151 3 View

Antigen retrieval for neural cell marker on frozen brain tissue?

Hi, I'm having problems with the Recombinant Anti-MAP2 antibody from Abcam (Recombinant Anti-MAP2 antibody [EPR19691] (ab183830)) antibody on my frozen, 4%PFA perfused, mice brain tissue. I...

10 January 2021 4,002 5 View