I have 3 groups which I would like to compare inbetween. I would like to compare them based on their gene expression profile (at the first place) and if possible also miRNA and lncRNA profile (depending on budget). Each group consist of 5 samples, so I have to run 15 samples alltogether.

I have two option,either to do NGS, on nexSeq500 by Illumina or affymetrix based chips (GEP)

My questions are.

It is possible to run on NGS both (coding and noncoding-mainly miRNA) in same run?

when YES how many reads per sample do I need (are suffcieint) and which kit should I choose. 

when NO, I would go just for coding gene expression analysis, which kit would You suggest and how many reads per sample are sufficient.

thank you forward for any help

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