I have 3 groups which I would like to compare inbetween. I would like to compare them based on their gene expression profile (at the first place) and if possible also miRNA and lncRNA profile (depending on budget). Each group consist of 5 samples, so I have to run 15 samples alltogether.
I have two option,either to do NGS, on nexSeq500 by Illumina or affymetrix based chips (GEP)
My questions are.
It is possible to run on NGS both (coding and noncoding-mainly miRNA) in same run?
when YES how many reads per sample do I need (are suffcieint) and which kit should I choose.
when NO, I would go just for coding gene expression analysis, which kit would You suggest and how many reads per sample are sufficient.
thank you forward for any help