What mobile phase should I use (TLC) to separate aromatic hydrazone and aromatic hydrazine?

More Andrej Hoivik's questions See All

Can we prove that set of all nested continued fractions N^N and the set of positive real numbers R^+ are homeomorphic?

Let us have a general sequence a in N^N Question 1 If we simply insert that sequence into the nested fractions, then we get a0+1/(a1+1/(a2+1/(a3+...))) It seems that for any such sequence we...

11 September 2023 8,065 5 View

Bone imunohistochemistry sectioning for analysis?

Hello everybody! I need to section a bone for immunohistochemistry. We have classical paraffin and cryo histology. For any advice or protocol, I will be very thankful. Thank you.

09 January 2023 9,474 1 View

Any help with PCR that is not working at all?

Hi, I have a problem with not working PCR. I am trying to amply two fragments next to each other on chromosome with Q5 high fidelity polymerase. I had problem with amplification of both. I tried...

01 November 2021 3,980 9 View

Langmuir adsorption isotherm, linear or non-linear fit?

Hi, when constructing a Langmuir (or any other) adsorption isotherm form experimental data, is it better to use a linear form of the equation [1/qe=1/(Kl*qm*ce)+1/qm] with linear fit or use...

21 April 2021 3,579 11 View

Which Human Reference Transcriptome for RNAseq?

I am currently conducting a RNAseq experiment of different human blood cell populations. I decided to go with Salmon for quantification of the samples as it seems to be one of the best algorithms...

18 February 2020 8,437 3 View

How to compare marker expression of cells with different autofluoresence?

I my experiment I want to assess changes in monocytic cells by flow cytometry after a specific treatment. For example after treatment I want to compare CD14 expression of treated cells vs. control...

19 December 2018 763 10 View

Are there anybody experienced in analyzing the RTG/CT (x-ray, axial computed tomography) from elasmobranch species ?

We are conducting pioneer X-ray and compute tomography studies on chosen species of elasmobranches. Research so far included rough shark, Oxynotus centrina (L.) (Squaliformes: Oxynotidae),...

02 December 2016 5,810 7 View

Does anyone know what is the Minimal Clinically Important Difference for the Emotional Availability Scales (developed by Zeynep Biringen)?

We have performed an RCT study of the effect of a video feedback method in a Norwegian sample, and would appreciate to learn from other's experiences with the scales.

05 October 2016 7,432 3 View

LS-DYNA. MAT_PLASTIC_KINEMATIC, Definition of FS factor?

When using MAT_PLASTIC_KINEMATIC, there is a FS factor which is defined as: FS:=Failure strain for eroding elements. Where can I find this strain for various steels? I have an example where for...

13 July 2016 9,300 1 View

Cell-Based Bioassay | Any tips on increasing assay window?

Hi all, I wonder if there are any tips reg. increasing the assay window? We perform 4-fold dilutions for 10 points. Currently in our case, if the initial concentration is relatively high, the...

19 January 2016 2,511 3 View

How do I replace a file with a more recent version of a paper that was uploaded to ResearchGate?

The paper in question is "Interpolation of Nitrogen Fertilizer Use in Canada from Fertilizer Use Surveys". This paper was very recently published by Agronomy (MDPI). Agronomy has, in the last day...

07 August 2024 9,934 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

What is the best protocol for freezing the rat brain in preparation for protein detection by Elisa ?

What is the best way to freeze the rat brain - liquid nitrogen, Dry ice , isopentane - after isolation for later protein investigation by Elisa?

01 August 2024 1,722 1 View

What is the principle/mechanism behind aging of carbon (graphite) containing refractory mix for isostatically pressed refractories?

The resin bonded carbon containing refractories are aged before use. How time of aging is determined? And mechanism behind aging.

23 July 2024 3,205 0 View

Can Langmuir constant related to bonding energy (Kl) and Freundlich exponent (1/n) indicative of ads intensity show opposite trends with temperature?

The adsorbate is Arsenic and the adsorbent is a soil

18 July 2024 332 8 View

How to create parameter and coordinate files for ligand in amber when FATAL error (maybe due to duplicate bond specifications) happens?

Hello, I'm trying to create parameter and coordinate files for a drug (PRG-A01) found on the following page: http://www.probechem.com/products_PRG-A01.html I used the guide made by Michael...

17 July 2024 3,472 3 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Can anyone suggest what amount of powder I have to disperse in the solvent for taking the FTIR spectra?

I have to compare the FTIR of the two samples, one is in powder form and other one is the powder dispersed in IPA or water. Both are same material, so how the bond will change

09 July 2024 8,011 2 View

Why there are no peaks at downfield NMR of Cobalt and Nickel complexes?

I synthesized some paramagnetic cobalt and nickel complexes (expected to be Co(II) and Ni(II)) using a bidentate ligand - N of pyridine ring (aromatic) and O of P=O (aliphatic), and I obtained...

03 July 2024 3,410 4 View

Which is true nano nitrogen or nano urea?

Some scientists use nano nitrogen and some use nano urea, even though the process used the same for preparation. So, which is true nano nitrogen or nano urea?

28 June 2024 2,885 6 View