Which Human Reference Transcriptome for RNAseq?

I am currently conducting a RNAseq experiment of different human blood cell populations. I decided to go with Salmon for quantification of the samples as it seems to be one of the best algorithms...

18 February 2020 8,329 3 View

How to concentrate cytokines in supernatant for ELISA?

I have problem to detect different cytokines in supernatant after the treatment of cells with LPS or TNF alpha by ELISA. Can you suggest me the way for concentration cytokines in cell supernatant...

01 May 2019 6,199 8 View

How to compare marker expression of cells with different autofluoresence?

I my experiment I want to assess changes in monocytic cells by flow cytometry after a specific treatment. For example after treatment I want to compare CD14 expression of treated cells vs. control...

19 December 2018 687 10 View

Cell-Based Bioassay | Any tips on increasing assay window?

Hi all, I wonder if there are any tips reg. increasing the assay window? We perform 4-fold dilutions for 10 points. Currently in our case, if the initial concentration is relatively high, the...

19 January 2016 2,406 3 View

Absolute quantification qPCR - alternative splicing problem. Can you lend your expertise?

I have a problem with absolute quantification and standard deviations. When I do my standard curve (with gBlocks) and run all my samples the standard deviation between triplicates are around 0,1...

15 July 2014 7,091 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

Can anyone share his experience regards Smoothing in origin?

I am working on TiO2 composite material and the results of XRD have a lot of small/junk peaks and while I apply a smooth filter in Origin then the other characteristics peaks disappear so anyone...

26 February 2021 8,183 3 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

I have need of XRD graph analysis software for phase identification. Can you please provide links for software tools which can be downloaded?

i am trying to download x'pert high score software, but can't download it. please do suggest software for phase analysis

24 February 2021 5,687 4 View

Why the CHi^2 is close to zero with a bad fitting but the Rp and Rwp are still high?

I am refining my XRD data using in FullProf program. The intensity of the modulated X-ray peaks is almost flat. I tried to adjust the peak intensities but unsuccessful. What is the reason? I guess...

23 February 2021 2,304 4 View

Can't recognize exact phase and PDF card no of Silicon carbide in XRD?

We have deposited silicon carbide on carbon substrate using one PVD technique. Due to amorphous behavior of SiC at low deposition temperature, we performed heat treatment of those films at...

22 February 2021 2,072 3 View