19 February 2020 3 8K Report

I am currently conducting a RNAseq experiment of different human blood cell populations. I decided to go with Salmon for quantification of the samples as it seems to be one of the best algorithms at the moment.

I am currently at the point where I have to build the index for the reference trancriptome. I did a lot of online research to find out which reference would be the most suitable for my project but I'm still not sure about it.

I was going to try:

Ensemble ( ftp://ftp.ensembl.org/pub/release-99/fasta/homo_sapiens/cdna/Homo_sapiens.GRCh38.cdna.all.fa.gz)

Gencode (ftp://ftp.ebi.ac.uk/pub/databases/gencode/Gencode_human/release_33/gencode.v33.transcripts.fa.gz)

Are these reference transcriptomes suitable for use with Salmon? If so, how do I decide which one is better for my setup? Which one is more frequently used?

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