Hello everybody!
I need to section a bone for immunohistochemistry. We have classical paraffin and cryo histology. For any advice or protocol, I will be very thankful.
Thank you.
Hope this helps.
Let us have a general sequence a in N^N Question 1 If we simply insert that sequence into the nested fractions, then we get a0+1/(a1+1/(a2+1/(a3+...))) It seems that for any such sequence we...
11 September 2023 8,065 5 View
Hi, I have a problem with not working PCR. I am trying to amply two fragments next to each other on chromosome with Q5 high fidelity polymerase. I had problem with amplification of both. I tried...
01 November 2021 3,980 9 View
Hi, when constructing a Langmuir (or any other) adsorption isotherm form experimental data, is it better to use a linear form of the equation [1/qe=1/(Kl*qm*ce)+1/qm] with linear fit or use...
21 April 2021 3,579 11 View
I have to measure by GC (MS of FID) different types of refrigerants (CFC) in gas samples without pure analyte or external standard in different mixtures. For example, R404a contains 3 different...
27 April 2020 9,996 5 View
I am currently conducting a RNAseq experiment of different human blood cell populations. I decided to go with Salmon for quantification of the samples as it seems to be one of the best algorithms...
18 February 2020 8,437 3 View
I my experiment I want to assess changes in monocytic cells by flow cytometry after a specific treatment. For example after treatment I want to compare CD14 expression of treated cells vs. control...
19 December 2018 763 10 View
Hydrazone (Nitrogen nitrogen double bond), hydrazine (single bond)
11 June 2018 6,256 2 View
We are conducting pioneer X-ray and compute tomography studies on chosen species of elasmobranches. Research so far included rough shark, Oxynotus centrina (L.) (Squaliformes: Oxynotidae),...
02 December 2016 5,810 7 View
When using MAT_PLASTIC_KINEMATIC, there is a FS factor which is defined as: FS:=Failure strain for eroding elements. Where can I find this strain for various steels? I have an example where for...
13 July 2016 9,300 1 View
Hi all, I wonder if there are any tips reg. increasing the assay window? We perform 4-fold dilutions for 10 points. Currently in our case, if the initial concentration is relatively high, the...
19 January 2016 2,511 3 View
I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...
07 August 2024 1,686 1 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....
29 July 2024 5,961 2 View
Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...
23 July 2024 6,314 3 View
Are both ways acceptable, or is there any convention to them? Thanks.
22 July 2024 6,988 4 View
Currently I need to calculate detection probabilities (PD) from radar cross section (RCS) data. Beta distribution parameters for this RCS data are calculated and will be used in Swerling0...
22 July 2024 868 0 View