What should the village government do in dealing with the spread of the covid-19 virus?

I am currently researching village government policies regarding the spread of the covid-19 virus, I want to gather opinions from various villages or areas affected by the Covid-19 virus, what...

07 September 2020 303 5 View

What are the importance of genetic tests to detect cancer?

Cancer is not a single disease with a single type of treatment. There are more than 200 different types of cancer, each with its own name and treatment. Medical tests can look for many inherited...

21 March 2019 209 5 View

How can PLGA be solidified while making composite with hydroxyapatite?

Hello people, I have been trying to develop composites from PLGA (Molar ratio- 50:50) with hydroxyapatite by solvent casting technique. But the slurry formed by mixing the two components does not...

27 December 2018 8,313 2 View

Why there is a decrease in the intensity and merging of xrd peaks of Hydroxyapatite after soaking it in SBF solution for 28 days?

I have prepared Hydroxyapatite pellets and immersed it in SBF solution for 28 days. The xrd analysis of post SBF Hydroxyapatite shows decrease in the intensity of peak and merging of some peaks...

20 November 2018 4,271 10 View

What are the challenges and outlook of biomass utilization in Metallurgical Industries?

One of the great challenges is to find a sustainable way to obtain biobased products from renewable resources. A significant emphasis has been on the developmental biorefinery concept and...

28 July 2018 2,346 3 View

How to calculate residual stresses for graphene using Raman sprectroscopy ?

After obtaining Raman spectroscopy of Graphene before and after composite sintering. The residual stresses in graphene to be calculated.

22 June 2018 1,863 3 View

What are the new improvements that make LED a powerful tool for lighting systems optimized for photosynthesis?

24 November 2017 9,935 5 View

HI, I need help to analyze the data obtained by in vivo recording of spontaneous activity from locus ceruleaus of mouse brain.

I would like to know in detail (step by step) how to analyze the data for spike detection and sorting using Axograph. Please find the raw data as attached. Thank you very much ! Please do to...

30 July 2017 6,075 1 View

How to identifying & charecterization of lactobacillus & Bifidobacterium? ??

25 July 2017 9,836 2 View

Bifidobacterium ::: how to isolate the colonies from capsules ?

17 July 2017 3,536 3 View

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02 March 2021 3,060 3 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

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28 February 2021 9,936 2 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View