Structure of the residual covariance matrix in a model with multilevel within-subject factors?

Hello, I have a dataset with a three-level structure. Participants reported an outcome of interest for 3-4 aspects of an incident, and they reported 4 types of incidents (1 incident for each...

09 February 2021 4,280 3 View

Mental health of COVID-19 Patients?

We want to conduct a study on the mental health and psychological problems in COVID-19 patients and would measure it in terms of Anxiety by Beck Anxiety Inventory, Depression by Patient Health...

02 February 2021 6,401 6 View

Which are the appropriate solvents for n-hexane plant extract for in vivo animal models to avoid toxicity?n-hexane plant extract is not soluble?

My n hexane plant extract is not soluble in any solvent. I have tried water, normal saline, tween 20 (2%, 5%, 10%, 50%), tween 80 (2%, 5%, 10%, 50%), pure DMSO, ethanol (2%, 5%, 10%, 50%, 97%),...

16 January 2021 3,547 3 View

Two antennas at transmitter and receiver both. How to limit signals such that each antenna at receiver get signal from only one antenna?

If I have two antennas at transmitter called T1 and T2 and signals are simultaneously transmitted from both antennas at same frequency towards two antennas at the receiver called r1 and r2. Is it...

16 January 2021 4,765 3 View

How much Morphine Standard is dire need for determination and extraction of Morphine in HPLC technique?

I want to purchase some Morphine Standard for HPLC to detect Morphine rate in Papaver somniferum L. how much is need in every stage or phase?

08 January 2021 1,675 3 View

What are the pros and cons of the Non-orthogonal multiple access (NOMA) scheme on Future Communication Networks?

21 December 2020 9,650 3 View

How Can I convert OD550 (Optical Density at 550 nm) to NTU (Nephelometric Turbidity Unit)?

OD and NTU are two different units, that can be measured through a spectrophotometer and Bioreactor (integrated with turbidity sensor) respectively. Because of the difference in units, the results...

03 October 2020 5,662 2 View

Can COVID-19 trigger diabetes?

06 September 2020 3,017 19 View

Is it possible to get higher wear rate with lower coefficient of friction?

i working on the enhancement of wear resistance properties of coated stainless steal in the absence and presence of various lubricant. In experiments , in the presence of some lubricants, the...

06 September 2020 8,338 26 View

ANSYS FLUENT is only launching in parallel. Can somebody please help?

My fluent is not launching in series at all, even when I uncheck the parallel option and i also have no option for using series. Please help!!! Many thanks!

04 September 2020 4,199 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What degree of missing data in a particular case/participant results in Expectation-Maximisation no longer working?

I'm dealing with a mediation model and am using the PROCESS module in SPSS. Due to SPSS and PROCESS being limited in the imputation methods - being unable to handle multiple imputation - the other...

02 March 2021 4,362 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View