I am working on CAT25 microsatellite biomarker. The melt curve shows two peaks while single band appears on the gel. Primers are taken from the published research papers. The DNA is of healthy individual. The umelt/quartz derivative plot also shows two peaks. The results of HRM and gel are attached. What could be the most likely reason of two peaks?

More Saadiya Zia's questions See All
Similar questions and discussions