Asima Zia

4 Questions 6 Answers 0 Followers

Questions related from Asima Zia

Can anyone have a good protocol for isolation of CD138+ plasma cells from human bone marrow samples using FACS?

I am going to work with multiple myeloma bone marrow samples. I need to isolate the CD138+ plasma cells. The sample will be processed the next day due to logistic reasons. Can anyone share their...

02 June 2023 10,004 0 View

Why did my mESc are floating and dead?

Hi, I recently cultured the AES0186-BRC-6 Feeder free mESc. When I cultured the cells, they were good until the second passage, and at the third passage, the cells died. I used the following media...

31 May 2021 8,990 1 View

What is wrong with these MEFs?

Hi, I am trying to culture MEFs to make a stock of Feder cells. These cells were purchased from Biolabs. Unfortunately, most of the cells died, and only a few were able to attach and were...

06 February 2021 7,106 3 View

MEF's didn't expand?

Hi I am trying . to grow and expand mouse embryonic fibroblast (MEF) from ATCC (SCRC-1008). I have worked with human cell lines, but this was my first time using the mouse cell line. I followed...

15 September 2019 5,031 2 View