Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Oocyte cultivation media - where can I find out about them?

Hello, can someone help me? I am just random undergrad interested in oocytes and early embryos but I know almost nothing about their in vitro cultivation. What is the current state of the art of...

23 August 2020 1,817 1 View

Can I pipette my small cDNA amount touching the wall of the tube? Can I sterilise my plastic well-plate by UV before using?

Hi, I have some questions about qPCR mix preparation. 1) If I am pipeting a small volume (0,5 microliter) of my cDNA after adding master mix, I draw off the liquid to the wall of the PCR well, not...

03 August 2020 3,940 4 View

Differentiation of mouse HSCs into various immune cell types?

The differentiation protocol of mouse CD34+ bone marrow cells into dendritic cells is well known. Is anybody familiar with protocols describing the differentiation of mouse CD34+ BM cells into...

16 September 2018 7,207 3 View

Exists a bioimpedance model/software of whole human body for current and voltage distribution for 4-electrode measurement?

Exists a bioimpedance model/software of whole human body current and voltage distribution simulation for 4-electrode measurement (similar to DOI10.5617/jeb.173)? Preferable for matlab use where...

28 March 2018 5,014 3 View

Can graphene be used in medicine?

My question is caused by a dual voice of research papers I have read. On one hand graphene or it's derivatives is used in composites with potential medical applications (stents, implants). I've...

20 March 2016 5,666 4 View

What is graphene foam?

06 January 2016 2,966 2 View

What is the maximum number of pure (95%) CD4+CD45RA+ naive T cells from one buffy coat?

Isolation of CD4+CD45RA+ T cells with high purity is essential for immune functional assays. Can you please share your experience with isolation of CD4+CD45RA+ (using Miltenyi isolation kits),...

03 December 2015 2,638 5 View

Does anyone have information where to purchase mice with progeria?

Looking for mouse models with progeria. Does anyone know where to purchase them?

15 August 2013 2,322 5 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

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01 March 2021 3,905 5 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Which group formation theory do you recommend for projects that aim to bring together entrepreneurs for training for regional development?

I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...

28 February 2021 4,223 2 View