Urban Švajger

12 Questions 6 Answers 0 Followers

Questions related from Urban Švajger

Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

28 October 2020 2,315 2 View

Isolation of human Th1 effector cells?

Is anybody familiar with an approach for direct isolation of Th1 effector CD4+ T cells from human peripheral blood? Perhaps in terms of sorting or via cytokine capture? Thank you for any suggestions!

25 February 2019 9,097 2 View

Differentiation of mouse HSCs into various immune cell types?

The differentiation protocol of mouse CD34+ bone marrow cells into dendritic cells is well known. Is anybody familiar with protocols describing the differentiation of mouse CD34+ BM cells into...

17 September 2018 7,385 3 View

CD80 and CD86 expression on monocytes

Is anyone familiar with pattern of CD80/CD86 expression on monocytes before and after stimulation with various stimuli e.g. LPS, IFNg, LPS/IFNgamma In my experience, CD80 was highly expressed on...

17 August 2017 4,911 4 View

Is repetitive/multiple transfusion of fresh/frozen plasma safe?

Can anybody please provide references on safe use or practice in repetitive transfusions of fresh (frozen plasma)? What are the cases where such procedures are most often used? Tnx!

11 May 2016 4,643 6 View

What is the maximum number of pure (95%) CD4+CD45RA+ naive T cells from one buffy coat?

Isolation of CD4+CD45RA+ T cells with high purity is essential for immune functional assays. Can you please share your experience with isolation of CD4+CD45RA+ (using Miltenyi isolation kits),...

04 December 2015 2,793 5 View

How many NK cells can I expect to obtain from one buffy coat?

Does anybody have experience regarding immunomagnetic isolation of NK cells from human buffy coats(cca 1x10e9 PBMCs)? What is the maximum number of cells one can obtain by microbead-selection? Tnx!

06 May 2015 5,064 6 View

What is the best timing for peptide-loading of DC vaccines?

What is the best timing to pulse dendritic cell vaccines with TAAs-peptides? How many hours before inducing maturation? I have also noticed some conduct peptide pulsing during the process of...

04 December 2014 9,229 5 View

Does anybody have any experience in which medium is best for MSC expansion when using either platelet lysate or human AB serum as substitutes?

Is it better to use DMEM-low glucose or alpha-MEM?

14 November 2014 3,278 6 View

Can anyone advise me which companies make good quality, GMP grade melanoma associated peptides for dendritic cell loading in vaccine preparation?

Are there any good companies in Europe?

06 November 2014 2,564 1 View

Does anybody have any experience and advice regarding CD40L for dendritic cell stimulation?

I am looking for the best and most practical way to stimulate/activate DCs via CD40L stimulation. The most practical seems soluble recombinant human CD40L (2 ug/ml) - however, it does not seem to...

17 September 2014 863 8 View

Does anyone have information where to purchase mice with progeria?

Looking for mouse models with progeria. Does anyone know where to purchase them?

16 August 2013 2,476 5 View