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Questions related from Urban Švajger
When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...
28 October 2020 2,222 2 View
Is anybody familiar with an approach for direct isolation of Th1 effector CD4+ T cells from human peripheral blood? Perhaps in terms of sorting or via cytokine capture? Thank you for any suggestions!
25 February 2019 9,042 2 View
The differentiation protocol of mouse CD34+ bone marrow cells into dendritic cells is well known. Is anybody familiar with protocols describing the differentiation of mouse CD34+ BM cells into...
17 September 2018 7,305 3 View
Is anyone familiar with pattern of CD80/CD86 expression on monocytes before and after stimulation with various stimuli e.g. LPS, IFNg, LPS/IFNgamma In my experience, CD80 was highly expressed on...
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Can anybody please provide references on safe use or practice in repetitive transfusions of fresh (frozen plasma)? What are the cases where such procedures are most often used? Tnx!
11 May 2016 4,604 6 View
Isolation of CD4+CD45RA+ T cells with high purity is essential for immune functional assays. Can you please share your experience with isolation of CD4+CD45RA+ (using Miltenyi isolation kits),...
04 December 2015 2,736 5 View
Does anybody have experience regarding immunomagnetic isolation of NK cells from human buffy coats(cca 1x10e9 PBMCs)? What is the maximum number of cells one can obtain by microbead-selection? Tnx!
06 May 2015 5,024 6 View
What is the best timing to pulse dendritic cell vaccines with TAAs-peptides? How many hours before inducing maturation? I have also noticed some conduct peptide pulsing during the process of...
04 December 2014 9,167 5 View
Are there any good companies in Europe?
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Looking for mouse models with progeria. Does anyone know where to purchase them?
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