Whis HLA-A2 TAAs are best for DC pulsation and CD8+ cytotoxic T cell induction?

When looking to establish Ag-specific CD8 cytotoxic T cells with autologous dendritic cells, there are numerous tumor-associated Ags to choose from. Does anybody know which Ags give the best...

27 October 2020 2,068 2 View

Oocyte cultivation media - where can I find out about them?

Hello, can someone help me? I am just random undergrad interested in oocytes and early embryos but I know almost nothing about their in vitro cultivation. What is the current state of the art of...

23 August 2020 1,817 1 View

Can I pipette my small cDNA amount touching the wall of the tube? Can I sterilise my plastic well-plate by UV before using?

Hi, I have some questions about qPCR mix preparation. 1) If I am pipeting a small volume (0,5 microliter) of my cDNA after adding master mix, I draw off the liquid to the wall of the PCR well, not...

03 August 2020 3,940 4 View

Differentiation of mouse HSCs into various immune cell types?

The differentiation protocol of mouse CD34+ bone marrow cells into dendritic cells is well known. Is anybody familiar with protocols describing the differentiation of mouse CD34+ BM cells into...

16 September 2018 7,207 3 View

Exists a bioimpedance model/software of whole human body for current and voltage distribution for 4-electrode measurement?

Exists a bioimpedance model/software of whole human body current and voltage distribution simulation for 4-electrode measurement (similar to DOI10.5617/jeb.173)? Preferable for matlab use where...

28 March 2018 5,014 3 View

Can graphene be used in medicine?

My question is caused by a dual voice of research papers I have read. On one hand graphene or it's derivatives is used in composites with potential medical applications (stents, implants). I've...

20 March 2016 5,666 4 View

What is graphene foam?

06 January 2016 2,966 2 View

What is the maximum number of pure (95%) CD4+CD45RA+ naive T cells from one buffy coat?

Isolation of CD4+CD45RA+ T cells with high purity is essential for immune functional assays. Can you please share your experience with isolation of CD4+CD45RA+ (using Miltenyi isolation kits),...

03 December 2015 2,638 5 View

Does anyone have information where to purchase mice with progeria?

Looking for mouse models with progeria. Does anyone know where to purchase them?

15 August 2013 2,322 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

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03 March 2021 5,360 2 View

Is there any test to determine the cardiovascular risk of patients with obesity? from the perspective of obesity?

We found tests to determine cardiovascular risk taking into account lipid profile, blood pressure, age, history of diabetes, other laboratories. But when weight is involved, there is a paradox. I...

02 March 2021 6,036 1 View

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02 March 2021 3,060 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View