I'm interested in amygdala's cell culture, but I am not finding a protocol to dissect this brain area to do a culture.
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Qual o fator motivacional acerca da preparação dos professores, bem como ocorre a práxis educativa nas escolas. Em análise, o fazer educacional comtempla os ditames da educação contemporânea?
16 July 2024 8,652 3 View
I will polymerise Aniline on ITO glass WE, using 1M of H2SO4 and a potentiostat. However, I am not sure if I have to do the electrochemical polymerisation inside a hood. Is it necessary? Is the...
10 July 2024 2,937 3 View
Hey everyone, I've been working with GRIN lenses (Inscopix) for almost 1 year and still had no luck seeing strong fluorescence on them. When I look at them, I can see good blood flow and,...
08 July 2024 5,178 2 View
What do you think is the main advantage of cell membrane-camouflaged nanoparticles, according to your experience?
04 July 2024 6,902 2 View
Does anyone have the full text of this article? KELIKIAN H, RIASHI E, GLEASON J. Restoration of quadriceps function in neglected tear of the patellar tendon. Surg Gynecol Obstet. 1957...
08 June 2024 7,054 0 View
If you prepare drug-loaded nanoparticles but only obtain microparticles (~2,000-4,000 nm), PDI~0.2, how can you reduce the size to reach ~100 nm?
02 June 2024 2,693 4 View
How to know if two drug molecules interact with each other, the strength (and type) of interaction and the possibility to form nanoparticles?
02 June 2024 4,304 1 View
Hello everyone, I am starting a soil mapping project in a specific crop region, and my goal is to perform shotgun sequencing (Illumina NovaSeq) to identify microorganisms and potential genes. This...
24 May 2024 5,021 4 View
As keys in input, I used: %Mem=20GB %Nproc=16 %chk=etilantraquinona_professor_func=6-31G_Au_Norte.chk #B3LYP/GEN OPT FREQ=RAMAN Nosymm PSEUDO=READ SCF=TIGHT" **** 0 2 . . . . C O H...
17 May 2024 391 2 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View