Hi there,
I need to inject 300 mg/kg of acetaminophen drug into C57BL/6 mice intraperitoneally. As recommended IP injection volume is 1% of the bodyweight (eg. 200ul for 20 gm weight), so I was trying to make 40mg/ml or 50 mg/ml solution so that minimum drug volume can be injected. But APAP drug is not soluble in water or saline. I tried heating it upto 55oC but it dint get dissolved.
I have read literature it says 15-20 mg/ml stock solutions can be made in water or saline. But if I make stock solution of less than 40 mg/ml then I'll have to increase the injection volume (i.e., more than 1%).
I would like to know if I can inject more than the recommended injection volume into mice? Has anyone else also faced the same issues?
Please reply.
Thanks
Vertica
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