Vertica Agnihotri

8 Questions 4 Answers 0 Followers

Questions related from Vertica Agnihotri

How to transform tissue-treated cultureware to low-adherent plates?

Hi, I'm trying to differentiate iPSCs to Immune cells and looking for plates with low-attachment. I came across this reagent "Anti-Adherence Rinsing Solution" by stemcell technologies which is a...

22 November 2024 9,087 2 View

Can I inject more than the recommended volume of drug intraperitoneally in C57BL/6 mice?

Hi there, I need to inject 300 mg/kg of acetaminophen drug into C57BL/6 mice intraperitoneally. As recommended IP injection volume is 1% of the bodyweight (eg. 200ul for 20 gm weight), so I was...

03 November 2022 9,932 0 View

Can anybody suggest me commercially available albumin out kits for sample preparation for 2D GE?

I’m using human serum samples for proteomic analysis. Can anyone suggest me if using Agilent Multiple affinity albumin removal columns are suitable enough in case I have to proceed with 2D gel...

06 June 2018 2,286 2 View

Protocol for indirect elisa quantification of cytokines (IL-10, IFN-gamma) in serum/plasma?

hii there, I would like to inform you that I'm working on IL-10 and IFN-gamma cytokines. I want to quantify these proteins levels in serum. I have tried using ELISA for the same but not getting...

23 January 2017 9,146 3 View

Can anyone provide me protocol for Ultra Competent cells Preparation?

I want to prepare ultra competent cells for efficient transformation. Kindly provide me its protocol. Thank You.

28 March 2016 8,403 2 View

I am looking for oral cancer cell lines. Can anyone provide proper information?

hii everyone,I have to initiate my work on oral cancer cell lines but unfortunately I am not able to get it any where in India. I have contacted NCCS (Pune) as well for the same concern but they...

21 October 2015 3,657 3 View

How can v use STATA9 for odds ratio calculations?

hii,i am working on a retrospective study. i want to ask if i can calculate odds ratio and adjusted odds ratio from STATA9 software. thank you.

10 September 2015 3,123 5 View

How can I calculate adjusted odds ratio in graphpad software?

I am working on case-control studies and using graphpad software for its analysis. I have calculated crude odds ratio among cases and controls, but facing some problems while interpreting adjusted...

25 August 2015 6,768 2 View