How to determine number concentration of polymer solutions using viscosity experiment?

I have synthesized PNIPAM (Poly (N-isopropylacrylamide)) microgels, which are copolymerized with oligo-ethylene glycol based comonomers. I have to determine the exact number concentration for each...

19 February 2021 5,205 5 View

Why the battery connected on the load side for storing purpose is taking the volatge /current at whatever rating it is given in matlab simulation?

I am designing a DAB DC DC Converter and when I am connecting a battery block in Matlab and fix its specification lets say 350 V and 25 A, when I am giving input as 600V, Battery would charge to...

11 February 2021 3,110 1 View

How do I connect DAB DC DC Converter to a battery in matlab simulation ?

I am trying to model a DAB converter and I need to observe the power transfeered to the load side on varying the phase shift between two bridges, hence I couldnt take a constant load e.g...

09 February 2021 6,489 3 View

Stably transfected A549 cells are getting washed off during a plate based assay?

HI, I have transfected A549 cells with a plasmid of interest. The transfectants have been stably selected and clonally separated. I am doing a plate based fluorescence assay with the transfectants...

20 December 2020 1,222 4 View

Does the number of the response categories (points of the Likert scale) affects the reliability of the scale?

Does the number of the response categories (points of the Likert scale) affects the reliability of the scale. Actually, i think that when we use 5 point Likert scale (strongly agree, agree,...

15 March 2019 661 3 View

Does addition of polypyrrole into epoxy resin decreases its brittleness?

when i have added polypyrrole into epoxy resin, it increased the elongation at break. I think the reason behind this behavior is the brittleness decrement of epoxy. Please help by discussing on...

15 August 2018 204 3 View

What is the reason behind higher elongation and lower modulus after the inclusion of reduced graphene oxide within the epoxy resin?

I have incorporated five wt% of rGO filler within the DGEBA resin and all composition except one have higher modulus and lower elongation. But that 30 wt% composition has opposite characteristics.

18 June 2018 1,217 3 View

Can anybody suggest me commercially available albumin out kits for sample preparation for 2D GE?

05 June 2018 2,094 2 View

Can anyone please suggest some methods for GABA detection from bacterial sample ?

I have already tried TLC with my samples. Also suggest what resuspension buffer would be best for GABA detection.

22 April 2018 1,966 2 View

I am always getting a high ppm error in the LCMS run samples ?

Ours is a SCIEX 5600 Qtof machine. We are standardizing the methods. We are always getting very high ppm error and therefore no matching in library. I have attached my data file for reference. I...

26 February 2018 5,009 7 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View

I want to performe MALDI-TOF MS for bacterial identification, I would appreciate it if anyone can tell me the matrix solution preparation protocol ?

based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...

26 February 2021 6,799 3 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Why ICP-MS test for Teflon filters that collected PM2.5, show really high results in the millions of ug/g ?

I analyzed soil and ash samples using ICP-MS for 28 metals, then I resuspended those samples to collect PM2.5 particles in a filter using a selective inlet inlet. My only issue that Fe and Al...

13 February 2021 7,592 3 View

What influences inter-day reproducibility in UPLC-MS/MS analysis?

I was analyzing some metabolites in human plasma. While the intraday precision of these metabolites was acceptable ( less than 20%), the interday precision was quite large. So what influences...

13 February 2021 9,183 9 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

2.6 Drying gas flow controller cannot regulate the requested setting after a fixed amount of time?

We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...

21 January 2021 2,969 1 View

How to distinguish pure and adulterated honey using NMR spectroscopy?

What are the common adulterants in commercial honey? how to identify pure honey and adulterated honey with the help of NMR spectroscopy? Is there any other technique to check purity of honey?

20 January 2021 5,456 12 View