Can anybody suggest a suitable vector system for preparing genomic DNA library from yeast Debaryomyces hansenii?

19 December 2014 1 4K Report

I am interested in making a library of genomic DNA fragments of D. hansenii in a suitable vector that can be propagated in E. coli but can preferably be used as an expression vector in S. cerevisiae, so that any transformants can be directly identified.

I want to use the Sau3AI partial digestion and BamHI cleaved vector for cloning of genomic DNA fragments. Please suggest suitable vectors

Rui Filipe Ramos Santos

Hey

You can use the Drag and Drop vector-system.

You can find here the information:

http://www.ncbi.nlm.nih.gov/pubmed/15656971

http://www.euroscarf.de/search.php?search=&project=Projects&gene=drag%20and%20drop%20cloning%20Jansen&selectedProject=Projects

It does work very well in S. cerevisiae.

Regards

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