we used for this experiment§ Fluorescein isothiocyanate in 10 mg vial (FITC; Invitrogen F-1906)
I also have same question, but for HeLa cell, there are some difference I need to notice?
I have a experiment related mice. I have to mate them and weight the weights in babies in every day. My concern is if I touch the pups every day do mother still accept their babies for breast...
11 October 2023 2,807 0 View
I have a polymer film. I want to do the CV, GCD and EIS studies using a three electrode system. KOH is the electrolyte, Pt wire as the counter and calomel as the reference electrodes.
23 February 2023 6,245 3 View
I used dean stark trap... But refluxing is happening in dean stark trap at about 145oC.
24 March 2022 6,652 1 View
Can anyone suggest me a media for the stimulation of MICP on a laboratory scale? There are a lot of papers suggesting the use of an NBU (modified nutrient broth with urea and CaCl2) media for the...
25 January 2022 5,664 0 View
Can you suggest me some chromogenic agents for detecting Ca ions using spectrophotometry.
28 December 2021 6,247 4 View
What media should I use to Isolate Bacillus megaterium, Bacillus Agar or sheep's blood agar. If can, please mention why?
20 November 2021 3,025 5 View
Extracted DNA sample dissolves in 0.1 M sodium acetate. It shows slightly turbidity.
26 October 2021 479 6 View
For the asymmetric price analysis (farm gate price, wholesale price and retail price) do we need to consider inflation? Almost all research used nominal price, can't we use real price instead of...
06 September 2021 3,239 4 View
I need to identify the wholesale and retail prices of vegetables transmission (symmetric or asymetric ) behavior, but my dataset stationary according to the aforementioned test, so what's the next...
17 August 2021 1,241 0 View
1.There are different techniques available to identify the unit root or stationary/ non stationary such as DF, ADF,KPSS, DFGLF and PP etc. What is the best technique or method to identify the unit...
16 August 2021 5,214 4 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
I am trying to compensate my flow cytometry data. I have human tumor tissue, dissociated and stained with live/dead fixable::APC, EpCAM::FITC, CD45::PE. I also stained and measured single stain...
07 July 2024 5,614 5 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....
19 June 2024 2,680 1 View
During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.
29 May 2024 5,165 0 View