Is it possible to identfy the difference between Methyl alcohol, ethyl alcohol and ethyl acetate extract,if so how?

I am working in the column chromatography for extracting plant extracts in Methyl alcohol, ethyl alcohol and ethyl acetate extracts I got confused with the columns that what column I placed which...

31 July 2020 4,937 3 View

How do we apply a bending stiffness on to a wire model in abaqus?

I was trying to mimic the deflection of a cantilever beam in wire on to a model with a portion of the beam replaced by a spring which provides a bending stiffness. I gave a usual spring with...

01 July 2020 6,869 4 View

How to rectify the"DIsplacement Increment for contact is too big" warning in abaqus?

I am currently modelling a joint of a geodesic dome. I previously ran an analysis only with preload of bolts ignoring all contact surfaces of the shank of the bolt and other geometry . This time...

03 June 2019 4,811 5 View

How to fit multiple curves on same data using the curve fitting toolbox in matlab.?

I have a data set and I want to fit multiple curves (say polynomial of order 4, 5, 6 etc) to the same curve using the cftool. While using curve fitting toolbox, it allows to fit only one type of...

21 December 2018 9,333 1 View

Can chitosan metal (Ag and Au) nano composite encapsulate DNA?

I have done DNA encapsulation using chitosan nano particle. Also I found many referee for that. But i couldn't find any references for encapsulation of DNA with chitosan metal nano composite.

26 October 2017 6,883 3 View

How to get K1 and K2 from J-integral for mixed mode?

17 February 2017 4,486 3 View

Why do damaged cells give high fluorescence intensity with rhodamine 123?

16 March 2016 4,380 2 View

Can someone help me to identify this SAED pattern for silver nanoparticle?

16 December 2015 1,022 6 View

How do you apply a sinusoidal load in ANSYS APDL?

I would like to apply a sinusoidal load to a rectangular plate in ANSYS.

29 July 2014 3,284 4 View

Is there any way to export the finite element meshes created in ANSYS (Mechanical APDL) to ABAQUS?

Ansys to abaqus

08 May 2014 1,327 8 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the nutrient requirements for E.coli BL21 CodonPlu RIPL strain growing in M9 medium?

Hello. I want to isotopically label the my protein, and I express it in BL21 RIPL using M9 minimal media. It grows extremely slow. I inoculate the colony from a fresh M9 plate into 5ml M9 medium...

17 February 2021 2,852 2 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

How to measure 50uL of cells?

Hello, I am planning to do a transformation, and on the protocol it says "Scrape a 50 ml volume of yeast from the YPAD plate using a sterile inoculating loop or sterile toothpick". But I can...

14 February 2021 6,247 3 View

Cells not growing in large scale protein expression?

I am using KRX cell. I have grown my overnight cultures at 37 degrees. When I inoculate 1L TB media, my cells do not grow. I have also tried using 25 degree incubation of overnight cultures and...

10 February 2021 3,871 3 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

Can I use the Chlorophyll stability index (CSI) to calculate the stress potential of cyanobacteria that are under stress?

I want to calculate the stress potential of cyanobacterial cells that are inoculated with antagonistic bacteria. My results showed that there is a drastic reduction in the chlorophyll content of...

23 January 2021 331 3 View

How to revive bacteria from glycerol stock?

19 January 2021 7,025 4 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

Should you include the charge when writing out ions or ionic compounds?

I'm writing a chapter about the nitrogen cycle and discuss the different forms of reactive nitrogen such as NO3 and NH4. I also have some soil places where I discuss soil cations and others where...

22 December 2020 3,753 3 View